![](https://parts.igem.org/images/partbypart/icon_rna.png)
RNA
Part:BBa_K3001014:Design
Designed by: Sydnee Calhoun Group: iGEM19_Lethbridge_HS (2019-10-12)
Lsh crRNA targeting GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A direct repeat stem loop was added to allow the crRNA to interact with Cas13a Lsh. The target sequence needs to be specific for GFP to avoid off-target effects.
Source
Designed by Laura Keffer-Wilkes based off of designs by Munich 2017. Synthesized by IDT.